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Western blot interpretation

About Western blot

Since some Western Blot bands are specific to the LYME bacteria, if you have any one of these bands showing on your test, LLMDs consider this to be enough to diagnose a LYME infection.

Interpretation of the Western blot—More is not necessarily better.



IgG

IgG

IgM

IgM

Band

kDa

Band importance

Ma et al.

2 of 6

CDC

5 of 10

Ma et al

2 of 5

CDC

2 of 3

18

Thought to be specific





22

Thought to be specific





23-25

OSP-C highly specific





28

Not specific





30

Thought to be specific





31

OSP-A highly specific





34

OSP-B highly specific





37

Thought to be specific





39

Thought to be specific





41

Non-specific flagella





45

Non-specific





58

Non-specific





66

Non-specific





73

Non-specific





88

Thought to be specific





93

Thought to be specific





Engstrom found 2 of 5 bands to be highly sensitive and specific for Lyme disease (Engstrom 1995), while 46 of 66 symptomatic pediatric patients with a history of bulls eye rash and tick bite were negative by CDC criteria (Fawcett 1995 Rheumatology Symposia Abstract #1254.) The CDC criteria are intended only for surveillance purposes, not diagnosis. Many physicians interpret the Western blot based on the number and specificity of the patient’s bands. See also (Ma et al. 1989).